Assay Development

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Proof of Concept

How we support you through each stage:

  • Weeks 1-4: Optimisation of surface binding of capture molecule. For example, its orientation and density.
  • Weeks 5-9: Optimisation of electrochemical protocol with a broad range technique exploration.
  • Weeks 10-16: Optimisation of testing protocol to align to immobilisation and electrochemical optimisation protocols.
    Initial assay characterisation.
  • Weeks 17-21: Characterisation aligned to manufacturing for capture molecule and sensor (defined by your requirements).
  • Weeks 22-30: Assay performance with spiked or inactivated samples in chosen sample matrices (defined by your requirements).
assay development proof of concept diagram

Assay Optimisation

Additional and optional services we offer:

Signal Optimisation

Further electrochemical protocol optimisation.

Alternative Surface Chemistry

Exploration of alternative surface chemistries for capture molecule immobilisation to increase assay sensitivity.

Preincubation Assay Format

Investigation of assay amplification techniques (e.g. secondary antibodies) to enhance assay sensitivity.

Assay Validation

Full assay characterisation (standard assay characterisation expanding to cross reactivity with complex matrices) aligned to customer requirements.

Ad Hoc Consultancy

Access to wider iGii team for consultancy for development on Gii platform.

Key benefits that Gii-Sens provides for assay development

Time to result

Assay results in less than 5 minutes

Assay performance

Very high sensitivity, typically equivalent to ELISA

Compatible Assay types

Small molecule, enzyme, antibody, antigen, nucleic acid

Multiplexing capabilities

Multiplexed, multimodal assays

Data output

Qualitative or quantitative

Reduced raw materials cost

Dramatically reduced bill of materials (blockers, secondary antibodies)